Chloroquine treatment in huh7 cells

Discussion in 'Plaquenil' started by latexxx, 05-Mar-2020.

  1. Rill New Member

    Chloroquine treatment in huh7 cells


    Chloroquine has been extensively used in mass drug administrations, which may have contributed to the emergence and spread of resistance. It is recommended to check if chloroquine is still effective in the region prior to using it.

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    Some side effects of chloroquine may occur that usually do not need medical attention. These side effects may go away during treatment as your body adjusts to the medicine. Also, your health care professional may be able to tell you about ways to prevent or reduce some of these side effects. As expected, hypoxia-induced degradation of both p62 and Keap1 in Huh7 and Hep3B cells was blocked by reconstituted expression of KHK-C or treatment of the cells with chloroquine CQ, a lysosome inhibitor. Chloroquine showed no antiviral activity in the MDMs. Toremifene reduced virus by 1–1.5 log10 at a dose more than 20 μM. Chlorpromazine reduced MERS-CoV by 2 log10 and had a narrow therapeutic window and a high toxicity. Chloroquine, Chloropromazine, and loperamide were tested on Huh7 cells. The cells were treated 1-h prior to infection.

    The Centers for Disease Control and Prevention recommend against treatment of malaria with chloroquine alone due to more effective combinations. In areas where resistance is present, other antimalarials, such as mefloquine or atovaquone, may be used instead.

    Chloroquine treatment in huh7 cells

    PDF Chloroquine inhibits hepatocellular carcinoma cell growth in., The protein kinase activity of fructokinase A specifies.

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  4. Huh7 cells or HLF cells were treated with or without 10 μM sorafenib in the presence or absence of 50 μM chloroquine or 100 nM bafilomycin A1 for 12 hr. b. Photographs of fluorescence microscopy of punctate fluorescence of a transfected mRFP‐GFP‐LC3 construct in Huh7 cells after 12‐hr treatment with 10 μM sorafenib.

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    How long is chloroquine half life when I treat a cell line for an autophagy study. Does anyone know if 25 or 50 uM of chloroquine treatment could be toxic for the cells C2C12 cells or any. Chloroquine 25 μM suppresses MMP-9 mRNA expression in normoxia and hypoxia in parental MDA-MB-231 cells. Chloroquine has cell-, dose- and hypoxia-dependent effects on MMP-2, MMP-9 and MMP-13 mRNA expression. TLR7 and TLR9 inhibition using IRS-954 or chloroquine significantly reduces HuH7 cell proliferation in vitro. Chloroquine CQ treatment induces the apoptosis of liver cancer cells. HepG2 and Huh7 cells were treated with CQ for 72 h. A Apoptosis was determined by Annexin V-FITC/PI double-staining analysis. Caspase-3 activity was analyzed by FACS B and cleaved.

     
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    RA and Hydroxychloroquine How Effective is it for.
     
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